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IDENTIFICATION

The identification of protein can be performed on several types of samples :

- complex samples such as total protein extracts (tissues, cells, physiological fluids), sub-proteomes or interactomes, (e.g., immunoprecipitation, co-immunoprecipitation or pull-down eluates)
- synthetic or chromatographically purified proteins/peptides

Depending on the complexity and the buffer in which your proteins are solubilized, an adapted sample treatment will be applied (SDS-PAGE separation, desalination, fractionation, etc.)

Step of protein identification

  • Sample processing
  • Digestion of proteins
  • Peptide Desalting
  • LC-MS/MS injection
  • DATA ANALYSIS

Deliverable

  • List of identified proteins (Excel format)

In practice: don’t hesitate to contact us to learn more at medecine-p3s@sorbonne-universite.fr or at 01.40.77.81.32

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