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QUANTIFICATION

LC-MS/MS analyses allow relative quantification of all the identified proteins under the different biological conditions studied.

The quantification is usually performed on proteins without prior isotopic labeling: this is called label-free quantification.

Depending on the complexity of the samples, a fractionation will be proposed for further analysis of the proteome.

Step of protein quantification

  • Splitting (optional)
  • Protein Digestion
  • Peptide Desalting
  • LC-MS/MS injection
  • Quantitative data analysis

 

Deliverable

  • Differentially expressed and significant protein list (Excel format)

In practice: don’t hesitate to contact us to learn more at medecine-p3s@sorbonne-universite.fr or at 01.40.77.81.32

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