LC-MS/MS analyses allow relative quantification of all the identified proteins under the different biological conditions studied.
The quantification is usually performed on proteins without prior isotopic labeling: this is called label-free quantification.
Depending on the complexity of the samples, a fractionation will be proposed for further analysis of the proteome.
Step of protein quantification
- Splitting (optional)
- Protein Digestion
- Peptide Desalting
- LC-MS/MS injection
- Quantitative data analysis
Deliverable
- Differentially expressed and significant protein list (Excel format)
In practice: don’t hesitate to contact us to learn more at medecine-p3s@sorbonne-universite.fr or at 01.40.77.81.32